The Orthodox Cathedral of the Holy Spirit in Chernivtsi was built on the initiative of the first Bukovinian Metropolitan E. Gakman, modeled on St. Isaac's Cathedral in St. Petersburg, with a dome 46 meters high. The construction of the neo-Renaissance temple was carried out from 1844 to 1864, and in 1860 the facade of the building was rebuilt. In 1961, the cathedral was closed, its valuables were transferred to various museums, the wall paintings of the lower part were damaged, and the 19th-century murals of the upper part of the walls by Karl Jobson were miraculously preserved. At first, a warehouse was arranged in the cathedral, then an exhibition hall of an art museum.

In 1989, the Cathedral of the Holy Spirit was returned to the local Orthodox community, and its restoration was carried out. Vladyka E. Gakman is buried in the cathedral, a monument has been erected to him. The main shrines of the temple are the relics of the Holy Great Martyr Barbara, a portable oak cross with particles of the Life-Giving Tree and the miraculous icon of the Mother of God, brought from Cyprus.